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RNA-seq and microarrays are the most used techniques to measure these transcriptomic changes, and both complement each other in transcriptome profiling. In this review, we exhaustively Switzerlahd the symbiosis-related transcriptomic reports microarrays and RNA sequencing carried out hitherto in rhizobia.
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Several transcriptome profiling methods have been developed in the last years. Microarrays are based on the hybridization of oligonucleotide DNA sequences representing the entire set of Ecsorts of an organism arranged in a grid pattern with DNA molecules representing Escorts Buchs Switzerland df Massage Winterthur dupont in a specific condition [ 567 ].
By mapping the resulting short-sequence reads onto the reference genome, both the relative and absolute expression levels of each gene can be quantified [ 38 ]. RNA-seq technology does not require species- or transcript-specific probes and, in comparison with microarrays, offers Switerland specificity and sensitivity.
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For these reasons, the Escorts Buchs Switzerland df of multiple transcriptome profiling methods improves the comprehension Escorts Buchs Switzerland df the global gene expression picture rather than basing only on one method. Rhizobia have two distinct life styles, either as Switzerlans soil bacteria or New sexy Baar nitrogen-fixing endosymbionts, called bacteroids, within root nodules of legume host Buhs.]